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Premier Biosoft beacon designer 8 software
Beacon Designer 8 Software, supplied by Premier Biosoft, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/beacon+designer+8+software/beacon+designer+software/pmc12960495-65-45-49
Average 86 stars, based on 1 article reviews
beacon designer 8 software - by Bioz Stars, 2026-10
86/100 stars

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Article Title: Metabolic and transcriptional responses demonstrating enhanced thermal tolerance in domesticated abalone.
Article Snippet: ⁎ Corresponding authors at: State Key Laboratory of Mari E-mail addresses: wwyou@xmu.edu.cn (W. You), chke@ 1 These authors contributed equally to this work. http://dx.doi.org/10.1016/j.scitotenv.2023.162060 Received 6 October 2022; Received in revised form 2 Available online 6 February 2023 0048-9697/© 2023 Elsevier B.V. All rights reserved.. • There were “winners” and “losers” in abalone during 20 years of high-temperature

Article Title: NKX2-1 gene is targeted by H19 lncRNA and is found to be overexpressed in benign nodular goiter tissues
Article Snippet: To eliminate residual genomic DNA, DNase treatment was carried out according to the manufacturer’s instructions (Thermo Fisher Scientific, Germany, Lot: 00645766). cDNA was synthesized using random hexamers as per the manufacturer’s instructions (Thermo Fisher Scientific, USA, Lot: 00645766). .. The exon-junction primers were created using Beacon Designer 8 software (Premier Biosoft USA). ..

Article Title: The Act of Diminishing the RTRAF Expression in Benign Thyroid Tissues Represents a Potential Method for Impeding the Progression of Malignancy in Cells
Article Snippet: .. Beacon Designer 8 software (Premier Biosoft, USA) was used for designing exon-junction primers. ..

Article Title: Development of genetically modified sweet cherry rootstock ‘Gisela 6’ with overexpression of PcMPK3-HA gene by Agrobacterium-mediated genetic transformation
Article Snippet: Genetic engineering is an efficient technique to improve target traits and provide a potential impetus for germplasm innovation of cherry rootstocks.. In this study, we used cherry rootstock ‘Gisela 6’ (Prunus cerasus × Prunus canescens) as the receptor for Agrobacterium-mediated genetic transformation and introduce the recombinant gene PcMPK3-HA into ‘Gisela 6’ rootstock.. In addition, we identified phenotypes during the formation of adventitious roots in transgenic lines.

Article Title: Selection and Validation of Reference Candidate Genes for qRT-PCR Analysis in the Developing Fruit of Phyllanthus emblica L.
Article Snippet: .. After determining the candidate genes, we designed primers in Beacon Designer 8 software (Premier Biosoft International) using the following parameters: the melting temperature (Tm) of the primers was 60 ± 1 ◦C, with a length of 18–21 bp and an amplification range of 80–150 bp. .. Primers were synthesized by Tsingke Biotech Co., Ltd. (Kunming, China).

Article Title: Functional Verification of Transcription Factor comp54181_c0 in Monascus purpureus.
Article Snippet: Monacolin K is a valuable secondary metabolite produced after a period of fermentation by Monascus purpureus; however, our current understanding of the regulatory mechanisms of its synthesis remains incomplete.. This study conducted functional analysis on the key transcription factor, comp54181_c0, that is involved in the synthesis of monacolin K in Monascus.. Mutant strains with either knockout or overexpression of comp54181_c0 were constructed using CRISPR/Cas9.

Article Title: Multiplex on-chip detection of Aspergillus by integrated ultrasonication-based bead beating lysis and magnetic beads direct amplification
Article Snippet: .. TaqMan primer and probe sequences with species-specific nucleotide targets ( C. neoformans : GenBank accession no. XM_770374 , A. fumigatus : GenBank accession no. CP097568 , A. niger : GenBank accession no. KX897144 and A. flavus : GenBank accession no. XM_041283833.1 )) were designed using Beacon Designer 8 software (Premier Biosoft International, Palo Alto, CA, United States). ..

Article Title: Multiplex on-chip detection of Aspergillus by integrated ultrasonication-based bead beating lysis and magnetic beads direct amplification
Article Snippet: .. We designed three primer-probe sets targeting A. flavus , A. niger , and A. fumigatus , along with a fourth set specific to C. neoformans , using Beacon Designer 8 software (Premier Biosoft, Palo Alto, CA, United States). ..

Quantitative RT-PCR:

Article Title: Development of genetically modified sweet cherry rootstock ‘Gisela 6’ with overexpression of PcMPK3-HA gene by Agrobacterium-mediated genetic transformation
Article Snippet: Genetic engineering is an efficient technique to improve target traits and provide a potential impetus for germplasm innovation of cherry rootstocks.. In this study, we used cherry rootstock ‘Gisela 6’ (Prunus cerasus × Prunus canescens) as the receptor for Agrobacterium-mediated genetic transformation and introduce the recombinant gene PcMPK3-HA into ‘Gisela 6’ rootstock.. In addition, we identified phenotypes during the formation of adventitious roots in transgenic lines.

Amplification:

Article Title: Selection and Validation of Reference Candidate Genes for qRT-PCR Analysis in the Developing Fruit of Phyllanthus emblica L.
Article Snippet: .. After determining the candidate genes, we designed primers in Beacon Designer 8 software (Premier Biosoft International) using the following parameters: the melting temperature (Tm) of the primers was 60 ± 1 ◦C, with a length of 18–21 bp and an amplification range of 80–150 bp. .. Primers were synthesized by Tsingke Biotech Co., Ltd. (Kunming, China).



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